Measuring 3D Cell Culture Viability in Multiple 3D Printed Scaffolds Within a Single Miniature Electrical Impedance Tomography Sensor

The use of 3D cell culture for tissue engineering and regenerative medicine applications often challenges conventional biochemical optical assays. Impedance-based cellular assays have shown their potential to retrieve dielectric parameters pertaining behavior such as viability, proliferation, differentiation 2D adherent culture. Herein, simultaneous impedance imaging viability measurements multiple large (>2 mm) cultures embedded in collagen gels are demonstrated. method is facilitated by low-resistance printed scaffolds that can hold a cell–gel system throughout while being transparent imaging. It silico vitro frequency-difference electrical tomography (fd-EIT) nondestructively label-free way differentiate variety concentrations with single miniature sensor real time. This study paves the toward development EIT quantitative noninvasive evaluation products.